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AIDS CLinical Reports
Military Academy Of Medical Science OF
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Test Report

Product Name: Gong Ming Anti-HIV Intravenous Injection Liquid (GMA-HIV)
Ref. No.:970301
Submitted By: Gong Ming Pharmaceutical Co. Ltd. (Heilong Jiang Province)
Test Purpose: Assess inhibitory effects of GMA-HIV on HIV-1
Submission Date: April 1997

Test Methodology

Setup

  • Different concentrations of GMA-HIV in a 96 well plate containing MT4 cells
  • Add 100 TCID50 HIV-1 suspension liquid
  • Conditions of Culture
    • 37<C
    • 5% CO2
    • 7 days
  • Study C GMA-HIV & MT4 cells in HIV-1 suspension liquid
    Control C MT4 cells in HIV-1 suspension liquidProcedure
  • Observe GMA-HIV toxicity effects on MT4 cells
  • Observe GMA-HIV inhibitory effects on HIV-1 mutagenic capability
  • Repeat the test 3 times
Test Results:
  • GMA-HIV at a concentration of 25 mg/ml is lethal to 50% of MT4 cells
  • GMA-HIV at a concentration of 12.5 mg/ml or greater can inhibit mutagenic effects of HIV-1 on MT4 cells
Conclusion: From this experiment, GMA-HIV has certain inhibitory effect (in vitro) on HIV-1.

Lab Technician: Wang, Hong Xia

Auditor: Li, Jing Yun

Date: 1997 May 15

People¨s Liberation Army Confirmatory Diagnostic Laboratory of AIDS